Like most organs, the gallbladder contains several different layers. The mucosa—that is, the innermost layer—consists of the epithelium and lamina propria, the latter of which is made up of loose connective tissue. The middle layer, the muscularis, plays a role in gallbladder contraction. The Rotitansky-Aschoff sinuses extend from the mucosal into the muscular layer. On the outside, a smooth membrane, the serosa, covers the organ.

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